Single-molecule technique to explain thermodynamic behavior of complex biomolecular systems

Most thermodynamic measurements of binding reactions rely on the validity of the law of mass action and the assumption of a dilute solution. Yet, important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not fulfill these assumptions and may require a particular reaction model. A new article reports that researchers have determined thermodynamic properties in biomolecular complex systems such as binding energies, chemical selecti…

Most thermodynamic measurements of binding reactions rely on the validity of the law of mass action and the assumption of a dilute solution. Yet, important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not fulfill these assumptions and may require a particular reaction model. A new article reports that researchers have determined thermodynamic properties in biomolecular complex systems such as binding energies, chemical selecti…